Tag Archives: stanford

What is in My Gut?

LesDethlefsenPostTop2

This past fall we learned about a unique study, conducted at Stanford University, designed to contribute to the understanding of the human microbiome. This study also has a component not common to academic research — data is being returned to the participants. Intrigued, I contacted the principle investigator, Les Dethlefson, to learn more.

Ernesto: Tell me about the Dynamics of Human Microbiota study.

les_dethlefsen_at_the_benchLes Dethlefsen: Since I joined the Relman Lab at Stanford, I’ve been looking at the human gut microbiota, focusing on what affects it and how it changes over time. In our study, we are looking at three different perturbations, deliberate changes to the gut ecology, to see how the microbiota population is affected.

We are very interested in the patterns that emerge. In people who have very stable gut microbiota, does their microbiota remain that way when they undergo diet shifts, a colon cleanout, or an antibiotic? Or maybe people who have a stable gut microbiota most of the time are the ones who are most affected by something unusual such as taking antibiotics. We just don’t know enough to understand these patterns right now. So, we’re really looking for basic ecological information.

Ernesto: If you look at the popular press, it seems the microbiome is the new golden child of biological life sciences. We’re even seeing companies in Silicon Valley get involved with this kind of work.

Les: It is broader than that. It really is a worldwide interest on the parts of both the scientific community and the public. And unfortunately, we are probably going to see some overhype, just as we did with the Human Genome Project. But I do believe this is a very important area. I think there will be a lot of payoffs and health impacts from this research, although it’s not going to be everything.

The shift that, I think, would be good for us to make intellectually is to get rid of the “us vs. them” thinking, because we are symbiotic organisms.

We have evolved with a native gut microbiota, and native microbiota is pretty much everywhere. We have evolved together, so it’s fallacious — an artifact of our past ignorance — that we don’t think of our microbes as part of our physiology.

Ernesto: It seems like exploring the deep sea, an unknown world that we’re just starting to peek into.

Les: It’s along those lines. You’re not wrong about that. But unlike, let’s say, the deep waters surrounding an undersea hydrothermal vent, we already know a lot about human physiology. There are a lot of molecular details and genetic pathways that we already have worked out. The context is somewhat understood.

And now, we have a reasonable start on the initial research: What microbes are present, and where? What’s the range of what we think is the normal distribution? We certainly don’t know enough, because we only know about people in the developed world. However, this may not represent all of human diversity or a very natural state of the gut microbiota.

Candida albicans

Candida albicans

Ernesto: Let’s get back to your study. You are asking participants to send microbiome data in the form of fecal matter and urine to your lab. What are you doing with those samples?

Les: We ask participants to provide both stool and urine samples. With the stool sample, we apply four different methodologies to turn it into data. One is the very common 16S ribosomal RNA (16S rRNA) gene sequencing approach. It’s relatively standard and inexpensive. It acts like an ID card for microbial taxa — telling us approximately what strains are present and in what relative abundance. We have a lot of data like that already for comparison.

sequencerThe second approach we will be applying is metagenomic sequencing, wherein we will be sequencing a random selection of all the genomes of the microbial types that are present. We can’t take this to completion, even with the dropping cost of sequencing, especially because there are some very, very rare microbes that we barely even have the chance to see at all. But we can get a pretty good swathe of genetic sequence data from all the microbes.

The third approach is even more ambitious. It’s called metatranscriptomics. Genes can be carried by any critter, you and I included, but not expressed. Knowing which genes are turned on, and to what extent they’re turned on is a better measure of the biological activity that is actually happening. The metagenome is a measure of potential activities, what the bugs can do. The metatranscriptome shows what the microbes are actually doing. Metatranscriptomics is even more challenging than metagenomics partly because of the nature of messenger RNA (mRNA). It’s a highly unstable molecule. There are technical challenges, but we’re ambitious enough to try to collect information on gene expression.

The fourth approach is not based on gene sequences, but on chemical composition. Metabolomics is the name given to a number of these approaches that are not directed to a specific chemical. These are techniques that try to measure a broad swathe of chemicals present in the environment and their relative abundance. This is a technology that we, in the Relman Lab, know very little about. We’re collaborating with the Nicholson Lab in Imperial College in London, and they will be doing the metabolomic analyses on the stool samples. That may be even closer to where the rubber meets the road — knowing not just the gene expression but also the resulting chemical changes that are happening in the environment.

MicrobeHeatMapMetabolomics takes us to the other type of sample we’re collecting: the urine samples. We aren’t doing this because we have an interest in the urinary microbiome itself, but because, as the Nicholson Lab suggested, the urine provides a more complete, integrated picture of the co-metabolism between the human host and most of the gut microbiota. So while metabolomics for the stool samples would primarily measure the gut microbial activity and what they contribute to the host’s physiology, the urine provides a more integrated picture about how the host metabolism works in concert with the gut microbiota.

Ernesto: If a participant is going to be contributing all of that data, will they have access to it?

Les: As someone with similar interests, I certainly knew that a huge motivation for people to join the study would be the access to their own data. We offer monetary compensation, but for the amount of time that will be spent in contributing samples, it is probably trivial. We knew we would attract the curious, scientifically inclined, and practising scientists. Of course, they would want to see their data.

The Institutional Review Board (IRB) was quite open to us sharing information with the participants about their own microbiota. It probably helped that there’s publicity  about ways people can get this information. There is the American Gut project, offering an assessment of your microbiota for a donation, and uBiome, a private company offering the same kind of service.

I, or another staff member of the study, are going to share this microbiota data with each participant in a conference call. So in effect, I’m going to be a microbiota counselor. It’s nowhere near as high-stakes as sharing genome information. We don’t know enough to say, for example, that this microbiome is definitively healthy, or that it’s unhealthy, or what the exact risks of diseases are due to this particular composition. So we will be putting this information in context, and we will be available as interpreters of the scientific literature. We may be able to say that there is a statistical association between a particular microbial group that someone may have in their gut and some health-related outcome.

sequence_data3

Microbiome sequence data

Ernesto: Will participants be getting a copy of their data as well?

Les: Yes, we will provide that. I have an open source mentality. Added to that is the fact that there are many practicing scientists signing up for the study and saying they want data, not just a PDF summary. I am happy to provide the data in as raw a format as people want. They can get the raw sequence information, a low-level summary (which is the result of the first pass of data processing), or the final summary. I have permission and full intention to share all the data derived from a person’s samples with that person.

Ernesto: Do you think we will see this happening more in the future?

Les: I think we will probably see more of it in the future. We’re moving in the direction of access to information. The open source movement has reached the health and medical realm from its origins in tech and computing. I think the participatory nature of access to data and scientific information is a good thing. It has started, and I don’t see any way of reversing the trend. I would hope that it becomes the norm that there is some appropriate level of sharing, that research participants have access to their data if they wish, and in a way that lets them interpret that data appropriately.

I believe that people have a right to that level of knowledge about their bodies, and if we, scientists, are generating that knowledge, there’s no reason not to share it with the individuals.


The Dynamics of Human Microbiota study is currenlty recruiting participants. If you’re interested in learning more about the ecosystem within read more about the study and check to see if you’re eligible to participate here.

We invite you to share your data access stories, and this article with the #qsaccess hashtag and follow along on quantifiedself.com and @quantifiedself.

 

 

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User Generated Health

This is a guest post from QS regular Chris Hogg. Thanks Chris!

Last week I had the great opportunity to see a series of demos of Lifelogging apps from students from Stanford’s CS247 Computer Human Interaction Design Studio.  I would really recommend checking out the demos here.  These are some great creative minds and future QSers (I hope).  It was pretty incredible to see what they were able to build in just 4-6 weeks.

Embedded below are slides from a guest lecture I gave to this class a few weeks ago entitled “User Generated Health”.  The theme of this talk was the value of user-generated data and its ability to reconnect us to our bodies and our health.  Those of you who have seen Gary Wolf’s TED talk will recognize that I completely ripped off his intro (flattery, right?), but i thought it was a really engaging way to ask the question of why we know more data about our cars than our bodies.  In the talk I also touch on a few companies who are proving that new data can be more valuable than a new drug, that Citizen Scientists have the power to disrupt the current clinical trial system and that the ultimate goal of personal data collection is self knowledge.

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Stanford Students Design for Lifelogging

Next Monday, Stanford students will show off their designs around lifelogging and mobile sensing. Here is the announcement from Jeff Heer, Stanford professor and Quantified Self advisory board member:

We’re having an action-packed year in the Stanford HCI program — in addition to celebrating the program’s 20th anniversary, we’re presenting a number of exciting new research projects at CHI 2011 and HCI courses are hitting record enrollments. Check out http://hci.stanford.edu for the latest.

Continuing the tradition, on Monday 3/14, 15 student teams in CS247 HCI Design Studio will be presenting their design explorations around the theme of “Lifelogging: mobile and online sensing for public or private good”. Come experience a variety of applications seeking to enhance our everyday lives – and meet top graduating students passionate about interaction design.

We will begin with food and socializing at 6pm at the d.school; short student presentations will commence promptly at 6:30pm, followed by a demo & poster session.

For more information and directions, visit:
http://cs247.stanford.edu/2011/show

Hope to see you there!
Jeff & the CS247 Staff

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How to Design for Behavior Change

How do I quit smoking, or start a running program? Drink tea instead of coffee, or eat more vegetables and less sugar? What about focusing on one task at a time instead of checking my RSS reader every 20 minutes?

If you are facing questions like this in your life or as part of your company, self-tracking can help bring awareness to patterns that you want to change. But what happens if awareness on its own is not enough to alter your behavior?

That’s where this guy comes in.

Continue reading

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QS Show&Tell #9 – Recap

stanford_arch.jpgThe QS Show&Tell #9 was very fun and interesting. Here is a quick recap with links.

We met at Stanford courtesy of Martha Russell of Stanford’s MediaX, and the evening began with Martha’s intro to her program, which links visionary research to industry applications. A list of fall seminars at MediaX shows a bit of what they are up to. The seminars are open to the public, and full of interesting things for QS types. Martha hosted as at the Wallenberg Hall Learning Theater, a great experimental learning space with high walls, three projection screens, and balcony viewing.

3banana.jpegSteve Brown’s presentation of 3banana came at a more fair and leisurely pace than at the last meeting, where he was stuck at the end of a long night of talks. Steve was the creator of Health Buddy, a pioneering self-tracking system, which he sold to Bosch Healthcare. 3banana is a more general tool, and Steve talked about his goal to augment human intelligence through giving us access to more efficient external memory. Steve’s talk showed a couple of self-tracking trends we’ve already noted coming together, including SMS as a tracking lingua franca and structuring data with hashtags.

Mark Carranza gave an update on his Social Memory Experiment, first presented at a QS last December. He is working toward taking his personal memory tracking system and releasing it as a social app, and invited all interested parties to help. More on Mark’s interesting work can be read here at this earlier post: The Social Memex.

MoodPHoneImage.gifWe got to meet Margie Morris, the inventor of the “mood phone” in person, as she was visiting the Bay Area.  Margie’s work was described in an earlier QS post: The Mood Phone and the Circumplex Model. Trained as a clinical psychologist, she is a senior researcher at Intel’s Digital Health Group. She played us some excerpts from videos with users discussing possible applications. One of the users in the video, a male working in a technical field, explained to the interviewer his practice of concealing his mood in order to avoid conflict, and speculated about the usefulness of a technical system to reveal actual emotional states. This provoked some interesting discussion of the social dimension of mood tracking.

alexandracarmichael.jpgAlexandra Carmichael invited everybody to take advantage of the stellar QS Scientific Advisory Board. These are professional researchers who are willing to field questions about self-tracking and self-experiment, which Alex will collect and transmit. The questions and answers will be published here, so that others can take advantage of the advice. Experiment design, statistical analysis, or other topics are welcome. Alex published her invitation on the blog a few weeks ago, in this post: Introducing the Quantified Self Advisory Board! Take a look at it and see the excellent resources available to you. (Video of her talk is here.)

Brian Mossop, who blogs at The Decision Tree, a blog about predictive medicine and the future of healthcare, presented an idea for a new smoking cessation company, that gave smokers a decreasing “budget” of cigarettes and rewarded them with permission to smoke cigarettes (withing the budget) when they met exercise or other goals. Brain’s father was a smoker, and he suspects that methods of self-tracking and simple rewards, plus some social encouragement, will be helpful to people trying to quit. (Video of his talk is here.)

Finally, Robin Barooah showed the results of his coffee and concentration self experiment. He posted here about it already. (See: The false god of coffee.)  His post was widely linked, with mentions on BoingBoing, Hacker News, and Freakonomics, and is now the most commented post on QS. Thanks Robin for a great post!

coffee making.JPG  

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